Study on distribution of YrY201-1 molecular marker among different stripe rust resistance cultivars of wheat

In this study,the DNA from 22 disease-resistant varieties,4 feeling cultivars and 12 wild relatives were used as templates and the distribution of molecular markers YrY201-1 of the stripe rust resistance gene YrY201 was speculated by PCR.The results showed that: the amplification of molecular marker YrY201-1 possessed high efficiency(96.2%,25/26).Target band could be amplified by this marker from all the 4 susceptible materials.The amplification efficiency of the 12 wild relatives was also very high(91.7%,11/12).And several non-specific bands except the target band were amplified from most of the materials.There was only one specific amplification band in the J11(Aegilops) which was the source of molecular markers YrY201-1 for stripe rust resistance gene YrY201,but slightly larger than the prospective band.