Study of xylooligosaccharides production by enzymatic hydrolysis with different materials and its composition analysis

The enzymatic property of xylanase and composition of enzymatic hydrolysates from bagasse,corncob,bran and brewer's grains were studied.The results showed that:the optimum temperature xylanase reaction were 60℃ and pH5.0.The activity of this enzyme was stable when the temperature was 40～60℃ and pH=5.0.Under the optimal conditions,bagasse,corncob,bran and brewer's grains were hydrolyzed by xylanase to produce xylooligosaccharides.The results showed as follows:the highest hydrolysis rate of xylan was bran with 21.19 mg/mL,followed by brewer's grains,corncob and bagasse.The analysis of enzymatic hydrolysates by HPLC indicating that the hydrolysates of the brewer's grains were mainly composed of xylobiose and xylotriose,reached to 13% and 26.7% respectively,and followed by corncob,bran and bagasse.