Cloning and Bioinformatics Analysis of Interleukin-of Sichuan White Goose

[Objective] The study aimed to clone interleukin-2(IL-2) gene from Sichuan white goose.[Method] Based on the IL-2 gene of duck accessed in GenBank,a pair of primers was designed for cloning IL-2 gene from total RNA of peripheral blood lymphocytes of Sichuan white goose stimulated by ConA via RT-PCR technology.The yielded fragment was sequenced for bioinformatics analysis.[Result] The whole length of IL-2 gene of Sichuan white goose is 468 bp that contains a 441 bp open reading frame(ORF),encoding 146 amino acid residues.Bioinformatics analysis shows that the amino acid sequence of IL-2 gene of Sichuan white goose contains four phosphorylation sites,a glycosylation site and a signal peptide with 21 amino acid residues.Homologies of IL-2 nucleotide sequence and amino acid sequence between Sichuan white goose and duck,chicken,turkey are 92.2%,77.5%,78.2% and 85.8%,65.5%,64.1%,respectively.By contrast IL-2 nucleotide sequence and amino acid sequence between Sichuan white goose and mammalian and rodents such as human,monkey,rat,bovine,horse,pig,cat,mouse,rabbit and deer,are all less than 45% and 28%,respectively.[Conclusion] The IL-2 gene of Sichuan white goose has closer genetic relationship with that of chicken and duck.