Detection of Toxoplasma gondii by Polymerase Chain Reaction(PCR) in Acute and Chronic Infected Mice

[Objective] The aim was to establish a high sensitive,specific and stable PCR technique for detecting T.gondii.[Methods] Detecting T.gondii in acute and chronic infected mice and evaluating the sensitivity of detection of T.gondii DNA in sera of acute infected mice.[Result] After inoculating for 18,21 and 24 h of acute infected mice,40%,80% and 100%,respectively infected mice were detected as positive.DNA of T.gondii could be detected in liver,spleen,lung,kidney and brain after inoculating for 18 d.In the test,we detected that one T.gondii was equal to DNA content of 0.2 pg T.gondii.[Conclusion] The results provide reference for the detection of T.gondii in mice by PCR.