2000•Journal of Zhangkai Aguotechaical CollegeRequires access
RAPD analysis on genetic diversity of mustard
Aimin Qiao
Open publisher page 0 citations
Abstract
Random amplified polymorphic DNA (RAPD) is a newly developed molecular marker technology. In this paper, RAPD markers were applied for analysis of the genetic variations among 16 mustard (Brassica juncea Coss.) varieties. 27 10 bp arbitrary primers were screened from 60. 336 DNA bands were amplified by the 27 primers, and among them, 275 were polymorphic ones, which accounted to 81.85% of the total. The average DNA bands amplified by each primer was 12.44. Genomic DNA fingerprints amplified by different primers were obtained. Most fingerprints had their characteristic bands or band patterns. The polymorphic percentage and the average DNA bands amplified by each primer were much higher than those of most studied species, which could be considered as the molecular foundation of rich genetic diversity in mustard.