Study on Factors Affecting the Browning During the Issue Isolation of Shiitake Mushroom

Taking mushroom as test materials,the browning probability of inhibiting tissue isolation by selecting different sizes of block,freshness Shiitake mushroom,water content medium,controlling the culture temperature and adding activated carbon and vitamin C into the culture medium,many factors that affecting the browning during the issue isolation were studied.The results showed that the browning rate was relatively low of 16.6% when the size of block was 0.7—0.9cm;while the rate was 0% when Shiitake mushroom was placed for 1—3days,and the culture medium was placed for 3days.The browning rate became 0% when adding 0.1%—0.3% activated carbon and 0.1—0.5g/L vitamin C.The browning rate was reduced to 16.6% when the temperature was controlled at about 25℃.The browning rate was the lower,the better the mycelia growth.