Expression and function of angiopoietin-1 during mouse kidney development

Purpose To observe expression parts and quantitative change of angiopoietin-1(Ang-1) during mouse kidney development in order to investigate the effect of Ang-1.Methods The kidneys of the foetus mice of different days and postnatal different days mice were taken.Kidneys of mice were used for observing the kidney development and the expression of Ang-1 by immunohistology and processed for Western blotting.Results The results of immunohistology showed that the expression of Ang-1 was faint at E14 and they were located in renal mesenchymal cells.Then the expression of Ang-1 tended to increase gradually following the kidney development and they were detected in glomeruli,blood vessels and renal tubules.The results of western blotting showed that the expression of Ang-1 protein increased gradually following the kidney development and peaked at 21 day,then decreased.Conclusion During mouse kidney development,Ang-1 was expressed in different region,and the intensities were evident.So it demonstrates that during the kidney development,Ang-1 is involved in nephrogenesis,growth and maturation.Simultaneously they have an important role in regulating and maintaining normal glomerular filtration.