2013•Nanfang shuichanRequires access

Stability comparison of reference genes of Pinctada fucata by real-time qPCR

Q Wang

Abstract

We analyze the expression stability of the housekeeping genes in Pinctada fucata,including Glyceraldehyde-3-Phosphate Dehydrogenase(GAPDH),beta-actin(β-actin) and 18S rRNA(18S),by quantitative real-time polymerase chain reaction(qRTPCR) in different tissues,at different stages of gonadal development and embryonic development.Besides,we select the suitable reference genes by using softwares of BestKeeper,geNorm and NormFinder algorithms.The results indicate that β-actin is the most stable reference gene for different tissues and different embryonic developmental stages,while 18S is the most stable reference gene for different gonadal developmental stages.

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Key concepts: Reference genes, Housekeeping gene, Biology, Glyceraldehyde 3-phosphate dehydrogenase, Gene, Real-time polymerase chain reaction, Gene expression, Cell biology