Direct prejection from diencephalon to lumbosacral spinal cord in rat

Objective To observe whether having the direct projections from the diencephalon to lumbosacral Spinal cord in rat and to put forward the morphological evidence to further research that diencephalon directly modulates the sacral parasympathetic nucleus.Method Using HRP retrograde tracing method, 5% WGA-HRP was injected into one side of 6th lumhara and Ist sacral segmentes in 20 rats,72 hours later, the rats perfused were killed and their brains were took out, diencephalon was sectioned by freezing. Sections were dyed with TMB method.Results The labeled neurous, in both side of the diencephalon but ipsilateral prominece, were found in the following nuclei; paraventricular nucleus, lateral area of hypothalamus,periventricular nucleus, anterior nucleus,arcuate nucleus, suprachiasmatic nucleus, lateral part of dorsomedial nucleus and posterio nucleus of the hypothalamus, parafascicular nucleus,subparfascicular nucleus and magnocellular part of ventrimedial nucleus of thalamus, Zona incerta and the Foral H2 field in subthatamus.Conclusion There are direct projectiones from diencephalon to lumbarosacral spinal cord in rat.