Experimental Studies on the Relation Between Serum Interleukin(IL-1,IL-6)and Acute Pancreatitis

Objective To study the relation between serum interleukin(IL-1,IL-6)level and acute pancreatitis.Methods 120 Wistar rats were randomly divided into 3 groups,40 each.The rats in normal control group were injected intraperitoneally with normal saline,rats in model group with acute pancreatitis were injected intraperitoneally with three doses of L-arginine at single dose of 500 mg/100 g body weight,with interval of one hour,and the rats in IL-10 treated group wre treated with 3 intraperitoneal injections of IL-10(10 000 u/rat),2,5,8 hours after L-arginine induced acute pancreatitis.All rats were killed 4,12,24,36 hours after L-arginine injection,respectively.Pancreas histopathologic evaluations and serum CRP,IL-1 aed IL-6 determination were then carried out.Results Histopathologic scores,and serum CRP,IL-1,IL-6 of the rats in model group with acute pancreatitis were significantly higher than those of the rats in normal control group,while those in IL-10 treated group were significantly lower than those of rats in normal control group,while those in IL-10 treated group were significantly lower than those of rats in the model group with acute pancreatitis.Direct correlation were found between serum interleukin(IL-1,IL-6)level and histopathologic scores(including CRP level)in the rats in model group with acute pancreatitis.Conclusion Serum CRP,IL-1,and IL-6 increase significantly in rats with serious acute pancreatitis induced by large dose of L-arginine injection.Treatment with IL-10 can significantly improve histopathologic changes in pancreas.Serum IL-1 and IL- 6 correlate directly with histopathologic changes in pancreas.