Agrobacterium Tumefaciens-mediated Genetic Transformation of Glycyrrhiza inflata Callus

Objective: A feasible scheme of Agrobacterium-mediated genetic transformation of Glycyrrhiza inflata callus was established in this paper,and the transformation conditions were also optimized. Method: Two agrobacterium strains of EHA105 and LBA4404 were used,pBI121-gfp plasmid was transformed in EHA105 and LBA4404 by the thermal shock method. Then Glycyrrhiza inflata callus was infected by Agrobacterium. Different preculture time of callus and different agrobacterium infecting time was setted as two conditions. After coculture,calli were estimated by fluorescence detection. Result: The results showed that green fluorescence protein was expressed in the calli. Screened by the medium containing 100mg / l Kan,the transformed calli were obtained. Conclusion: A Agrobacterium-mediated genetic transformation system of Glycyrrhiza inflata callus laid a foundation for further research on target gene transformation to the callus and regulation of the pathway of the secondary metabolites biosynthesis of liquorice via genetic engineering mothed.