Physiological and biochemical response of pea seedlings to endogenous and exogenous NO under salt stress

A pea variety‘longwan1'was used as the experimental material to study the effect of endogenous and exogenous NO on the growth of pea seedlings under NaCl stress with NO donor sodium nitroprusside(SNP),NO scavenger c-PTIO and sodium nitroprusside analogues sodium ferrocyanide.It was found that adding exogenous NO increased the plumule and radicle length,seedling dry weight,root-shoot ratio,chlorophyll content,soluble protein and sugar levels,and the activities of SOD,POD and CAT of the pea seedlings.The content of proline was also increased under salt stress.Meanwhile,exogenous NO also alleviated the accumulation of MDA and decreased the membrane relative permeability in leaves of pea seedlings under salt stress.Endogenous NO had a significant effect on growth of germ,seedling dry weight,soluble sugar content,and MDA,SOD and CAT activity.In summary,exogenous and endogenous NO both alleviated the damage to pea seedlings caused by salt stress.