Production of Rat Retina Frozen Section Used for Immunofluorescence Staining

Objective: To explore optimal frozen section method and technique for immunofluorescence staining of rat retina. Methods: Eyes of SD rats were taken with perfusion or not after anesthesia,and fixed and dehydrated according to the various specimen processing. A total of 60 frozen slices in different sickness were made,and then marked with immunohistochemical antibody. The cells arrangement and the status of the tag were observed with fluorescent microscope. Results: Full eye retina structure form could keep the relative rules,but organization pulling occasionally occurred. Immunofluorescence results of optic cup specimen after vitreous removal were relatively ideal. And the results of specimen thickness between 10- 12 μm were superior to others. Conclusion:Tissue samples for immunofluorescence should try to maintain its complete natural form by means of quantitative and qualitative,positioning of the target structure and reproduce style of the retina component.