Construction and Expression in Vitro of Eukaryotic Expression P1asmids of HBeAg

Objective To construct a vector expressing HBeAg in eukaryotic cells.Methods Amplifying the preC/C gene from the PHBV1.5 vector by PCR,the product of PCR was cloned into the pCDNA3.1,the recombinant vector was confirmed by restriction enzyme digestion,PCR and sequencing, then it was transfected into HeLa cells to ensure the expression of preC/C protein and HBeAg.Result The Results of the restriction enzyme digestion,PCR and sequencing confirmed the vector was constructed successfully,furthermore it can express preC/C protein and HBeAg in supernatant.Conclusion The vector was constructed successfully,it laid foundation for using RNA interference to suppress the expression of HBeAg.