A small interfering RNA targeting vascular endothelial growth factor inhibits CNE-2Z nasopharyngeal cancer cell neovascularization in vitro

[Objective] To study the inhibiting effect of vascular endothelial growth factor (VEGF) siRNA to the expression of VEGF in CNE-2Z Nasopharyngeal Cancer cells, and to explore the feasibility of VEGFsiRNA in treating nasopharyngeal cancer. [Methods] Nasopharyngeal squamous cells of human (CNE-2Z) were cultured and divided into the normoxia culture group (20% O2) and the hypoxia (1% O2) culture group. VEGFsiRNA were transfected into CNE-2Z cells of two groups with liposome (LF2000). VEGF mRNA expressions of normoxia and hypoxia were tested by RT-PCR and ELISA assay. [Results] VEGF mRNA was expressed in CNE-2Z cells cultured in normoxia. VEGFmRNA expression increased in hypoxia, and there is significant difference between the two groups (P 0.01). VEGFsiRNA downregulated VEGFmRNA expression significantly compared with that of non-transfection group and the vector transfection group (P 0.01) both in normoxia and hypoxia; Downregulation of VEGFmRNA expression in normoxia was higher than in hypoxia. [Conclusion] VEGF-specific siRNA can inhibit VEGF mRNA expression in nasopharyngeal squamous cell of human (CNE-2Z).