STUDIES ON THE ANTIOXIDATION ACTIVITY OF SQUID BODY HYDROLYSATES

Dilute homogenate was prepared with grinding squid body.The hydroxyl free radical scavenging activity(SA) of the squid dilute homogenate hydrolysate, which was produced by hydrolysis with four kinds of different protease, was investigated by examining the absorbance with 721 UV-Spectrometer.The result revealed that the radical scavenging activity of the hydrolysate increased significantly after the squid homogenate was hydrolyzed with different enzyme.The hydrolysates digested with Papain showed the highest scavenging activity among the four kinds of protease.The most suitable hydrolysis parameters of Papain, determined by the orthogonal test design based on the single factor experiment, include substrate protein concentration 4 %, enzyme /substrate ratio 18 000 U/g, temperature 65 ℃, pH=8 and time duration 0.5 h, with the scavenging activity 29.92 % of hydroxyl free radical.The variance analysis of affecting factor of scavenging activity on hydroxyl free radical indicates that temperature, pH, enzyme , substrate concentration all have great effect on antioxidation and have significant difference.