Change of Vascular Endothelial Growth Factor in Ovarian Hyperstimulation Syndrome Patients

Objective: To explore the role of vascular endothelial growth factor (VEGF ) in pathogenesis of ovarian hyperstimulation syndrome (OHSS) so as to provide experimental evidence for early diagnosis of OHSS. Methods:VEGF levels in serum on the day of hCG injection and in follicular fluid on the day of embryo transfer (ET) and oocyte retrival were measured by ELISA. The expression of VEGF mRNA of granulosa cells was detected by RT-PCR. Results: There was no significant difference between the OHSS and non-OHSS group on levels of VEGF on the day of hCG injection in serum (102. 5 ± 40.80 and 90.23 ± 34.62 pg/mL, P0. 05). There was significant difference between the two groups on levels of VEGF on the day of ET in serum (OHSS: 150. 93 ±59. 91 and non-OHSS 104.18 ± 20. 76 pg/mL, P 0, 01). There was significant difference between the two groups on levels of VEGF in follicular fluid (782. 86 ±136. 87 and 362. 50±231. 85, P 0. 05). The comparison of serum VEGF on the day of hCG injection and embryo transfer showed significant difference in the OHSS group, but no significant difference in the non-OHSS group. The results of RT-PCR revealed there was significant difference between the ratio of VEGF/β-actin in the OHSS group and the non-OHSS group (0. 54± 0. 24and 0. 27±0. 12, P0. 01). The expression of VEGF mRNA in the OHSS group was more obvious than that in the non-OHSS group. Conclusion; VEGF plays certain role in the pathogenesis of OHSS. Measuring VEGF levels in follicular fluid and serum on the day of embryo transfer can predict the occurrence of OHSS.