Quantitative Analysis of Emulsifier OP by Fluorescence Spectrophotometry

The relative strong fluorescence intensity was obtained in wavelength of 304nm by investigation on fluorescence spectra of emulsifier OP(p-octyl polyethylene glycol phenyl ether).There was a good linear relationship between fluorescence intensity and concentration of emulsifier OP in the certain range in B-R buffer solution with pH of 5.5,based on which a novel-high-sensitive determined method was established.In the concentration range of 0.1—50.0μg/mL,the regression equation for wavelength of λex/λem=226nm/304nm was F=22.12C+9.825(C:μg/mL,r=0.9995) with detection limit of 0.068 μg/mL,and the regression equation for wavelength of λex/λem=276nm/304nm was F=90.08C-10.12(C:μg/mL,r=0.9999) with detection limit of 0.053μg/mL.The same samples were determined by above two wavelengths,and all recoveries were above 96.5%,and the two results had no significant difference through F-test and t-test.This method can be applied to the determination of amount of trace emulsifier OP in complex environmental samples with advantages of high sensitivity and strong anti-interference capacity.