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2007•Chinese Journal of Clinical NeurosciencesRequires access

Culture and Differentiation of Neural Stem Cells from Spinal Cord of GFP-Transgene Embryonic Rat and Expression of Neuregulin-1

Jin Jin

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Abstract

Aim:To isolate and culture the neural stem cells(NSCs) from spinal cord of the GFP+-transgene embryonic rat,and to observe the multiple functions and Neuregulin-1 expression of NSCs. Methods:NSCs were isolated and cultured from spinal cord of the GFP+-embryonic rat(E16d) . The Neuregulin-1 expression of neurospheres and identification of cellular types differentiated from NSCs were determined by the method of immuno-histochemistry. Results:NSCs could be isolated and cultured from spinal cord of the GFP+-embryonic rat. The neurospheres could express Neuregulin-1 and Nestin,and it could further differentiate into neurons,astrocytes and olygodenrocytes. Conclusion:NSCs could be isolated and cultured from spinal cord of the GFP+-embryonic rat. The NSCs have multiple functions of which differentiate into various neurocytes used in the treatment of CNS diseases.

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What this paper is about

Aim:To isolate and culture the neural stem cells(NSCs) from spinal cord of the GFP+-transgene embryonic rat,and to observe the multiple functions and Neuregulin-1 expression of NSCs. Methods:NSCs were isolated and cultured from spinal cord of the GFP+-embryonic rat(E16d) . The Neuregulin-1 expression of neurospheres and identification of cellular types differentiated from NSCs were determined by the method of immuno-histochemistry. Results:NSCs could be isolated and cultured from spinal cord of the GFP+-embryonic rat. The neurospheres could express Neuregulin-1 and Nestin,and it could further differentiate into neurons,astrocytes and olygodenrocytes. Conclusion:NSCs could be isolated and cultured from spinal cord of the GFP+-embryonic rat. The NSCs have multiple functions of which differentiate into various neurocytes used in the treatment of CNS diseases.

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Available abstract

Aim:To isolate and culture the neural stem cells(NSCs) from spinal cord of the GFP+-transgene embryonic rat,and to observe the multiple functions and Neuregulin-1 expression of NSCs. Methods:NSCs were isolated and cultured from spinal cord of the GFP+-embryonic rat(E16d) . The Neuregulin-1 expression of neurospheres and identification of cellular types differentiated from NSCs were determined by the method of immuno-histochemistry. Results:NSCs could be isolated and cultured from spinal cord of the GFP+-embryonic rat. The neurospheres could express Neuregulin-1 and Nestin,and it could further differentiate into neurons,astrocytes and olygodenrocytes. Conclusion:NSCs could be isolated and cultured from spinal cord of the GFP+-embryonic rat. The NSCs have multiple functions of which differentiate into various neurocytes used in the treatment of CNS diseases.

Key concepts: Neurosphere, Embryonic stem cell, Neural stem cell, Nestin, Neuregulin 1, Biology, Spinal cord, Neuregulin

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