2013•Chinese Journal of Health Laboratory TechnologyRequires access

Detection of enterotoxin genes of enterotoxigenic Escherichia coli by duplex real-time fluorescence polymerase chain method

Hui Yang

Abstract

Objective:To develop a rapid,accurate,sensitive method to detect the enterotoxin genes of enterotoxigenic Escherichia coli.Methods: The sequences of enterotoxin genes lt and st of ETEC were searched and blasted,then primers and TaqMan-MGB probes were designed.The primers,probes,concentrations of Mg2+,Taq DNA polymerase and dNTPs level were modified.The specificity,sensitivity and repeatability were analyzed.Results: A duplex real-time PCR assay was developed for the detection of enterotoxin genes lt and st of ETEC.Conclusion: A sensitive and specific method for the detection of enterotoxin genes of ETEC was developed.This method has widely practical application value in disease prevention and clinical laboratories.

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Key concepts: Enterotoxin, TaqMan, Enterotoxigenic Escherichia coli, Polymerase chain reaction, Escherichia coli, Real-time polymerase chain reaction, Biology, Heat-stable enterotoxin