Study of Spontaneous Apoptosis and Celluler Proliferation Activity on Early Gastric Carcinoma

Objective To investigate the clinical pathological significance of the apoptosis and proliferation activity in human Early gastric carcinoma.Methods TUNEL(terminal uridine deoxynucleotide nick end labeling) and electron microscope were used to display apoptotic cells.Immunchistochemisty was used to detect the expression of PCNA and P53.DNA ploidy was determined by image analysis technigue.Results The total 60 cases of colorectal cancer were observed.AI and AI/PI in well-differentiated cancer were significantly lower than those in poorly differentiated cancer(P0.05).The deeper the invasion of the cancer,the lower the AI and AI/PI(P0.01) and the higher the PI and DNA5C(P0.05).P53 negative staining cases had higher AI(P0.05).Conclusion Well-differentiated,early or mid-term stage and small size fumours have higher AI and AI/PI.AI is negatively correlated with PI.PI is correlated with node metastasis.It indicated proliferation is increased and apoptosis inhibited with development of the cancer.P53 mutation can significantly decrease the apoptosis of colorectal cancer cells.