Experiment on Electrospun PLGA-Collagen Fibers for Skin Tissue Engineering Scaffolds

To prepare the scaffolds of excellent biocompatibility for skin tissue engineering.PLGA and collagen were used to make the skin tissue engineering scaffold by electrospinning.The physical properties were observed,such as morphology,degradation and the biocompatibility by CCK-8 and HE stainning.The fiber scaffold had good morphology and uniform diameter.The mass loss of scaffold was 35% after degradated 8 weeks in phosphate buffer,which suggested that it had ideal biodegradability.Human fibroblasts could grow well on the scaffold.After implanted 3 d,fibroblasts' relative viability was 75%,and after 14 d,relative viability was 90%.This result showed that there were no obvious cytotoxicity.The electrospun PLGA-Collagen scaffold is fit for skin tissue engineering and has the potential to build a biological skin tissue engineeirng.