Effect of Scutellaria barbata Extract for Survivin Protein Expression in Human Lung Cancer A549 Cell

Objective:To investigate the effect of Scutellaria barbata extract for survivin protein expression in human lung cancer A549 cell and its mechanism.Method:Using the MTT assay to display the sensitivity to the drug,the inhibition of S.barbata extract to lung cancer cell(A549,10% fetal calf serum,1640 nutrient medium)in vitro was observed.TUNEL was used to test the apoptosis,immunocytochemistry was applied to examine the expression of survivin protein.Result:The inhibition rate of S.barbata extract(5~40 μg·mL-1)to A549 indicated a dose-dependent anti-proliferative effect by MTT assay(P0.01).The apoptotic index was(38.40±7.41)% in experiment group(S.barbata extract 18.86 μg·mL-1),higher than that(6.22±0.45)%in control group,showed a significant difference(P0.01).Compared with control group,the masculine cells of survivin protein was decreased in the experiment group obviously(P0.01).Conclusion:The proliferation of A549 cell was inhibited obviously by S.barbata extract and the mechanism maybe related with survivin expression downregulation.