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A research on the effects of matrix metalloproteinase-2 and tissue inhibitor-2 of metalloproteinase expression on atrial structural remodeling in patients with atrial fibrillation caused by rheumatic heart disease

Wei He

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Abstract

Objective To investigate the matrix metalloproteinase-2 (MMP-2) and tissue inhibitor-2 of metalloproteinase (TIMP-2) mRNA expression in patients with atrial fibrillation caused by rheumatic heart disease and to evaluate the influence of MMP-2 and TIMP-2 expression on the progress of atrial structural remodeling.Methods 42 patients with chronic atrial fibrillation(AF) caused by mitral valve straitness of rheumatic heart disease were as experimental group, and l8 patients with sinus rhythm were as control group.Before operation,all of the above patients underwent transthoracic echocardiograph,and related clinical date were collected. Left atrial appendage (LAA) tissue samples were obtained from these patients during mitral valve replacement operation.Collagen fibers in left atrial appendage were observed by Masson trichrome stain. MMP-2 and TIMP-2 mRNA expressions were determined by reverse transcription polymerase chain reaction (RT-PCR).Results (1)Compare with control group,the content of collagen fibers in LAA increased significantly in patients with AF(P0.01). The content of collagen fibers in LAA was significantly correlated with left atrial dimension(r=0.788,P0.001)and the aera of mitral valve(r=-0.886, P0.05). (2)Compared with control group,the expressions of MMP-2 mRNA(P0.05) in the LAA tissue of the AF group was up-regulated significantly;the expressions of TIMP-2 mRNA(P0.05) in the LAA tissue of the AF group was down-regulated significantly. (3)The expressions of MMP-2 mRNA was significantly correlated with TIMP-2 mRNA(rp=-0.766,P0.001). The MMP-2 was significantly correlated with the content of collagen fibers in LAA(rp=0.869,P0.001).The TIMP-2 was significantly correlated with the content of collagen fibers in LAA(rp=-0.830,P0.001).Conclusion The selective down-regulation of TIMP-2 and up-regulation of MMP-2 gene expression in atrium,especially the unbalanced change of the MMP-2 and TIMP-2 gene expression,could be one of the molecular mechanisms of atrial structural remodeling in patients with atrial fibrillation caused by rheumatic heart disease,which correlates with the initiation and maintenance of AF.

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Objective To investigate the matrix metalloproteinase-2 (MMP-2) and tissue inhibitor-2 of metalloproteinase (TIMP-2) mRNA expression in patients with atrial fibrillation caused by rheumatic heart disease and to evaluate the influence of MMP-2 and TIMP-2 expression on the progress of atrial structural remodeling.Methods 42 patients with chronic atrial fibrillation(AF) caused by mitral valve straitness of rheumatic heart disease were as experimental group, and l8 patients with sinus rhythm were as control group.Before operation,all of the above patients underwent transthoracic echocardiograph,and related clinical date were collected. Left atrial appendage (LAA) tissue samples were obtained from these patients during mitral valve replacement operation.Collagen fibers in left atrial appendage were observed by Masson trichrome stain. MMP-2 and TIMP-2 mRNA expressions were determined by reverse transcription polymerase chain reaction (RT-PCR).Results (1)Compare with control group,the content of collagen fibers in LAA increased significantly in patients with AF(P0.01). The content of collagen fibers in LAA was significantly correlated with left atrial dimension(r=0.788,P0.001)and the aera of mitral valve(r=-0.886, P0.05). (2)Compared with control group,the expressions of MMP-2 mRNA(P0.05) in the LAA tissue of the AF group was up-regulated significantly;the expressions of TIMP-2 mRNA(P0.05) in the LAA tissue of the AF group was down-regulated significantly. (3)The expressions of MMP-2 mRNA was significantly correlated with TIMP-2 mRNA(rp=-0.766,P0.001). The MMP-2 was significantly correlated with the content of collagen fibers in LAA(rp=0.869,P0.001).The TIMP-2 was significantly correlated with the content of collagen fibers in LAA(rp=-0.830,P0.001).Conclusion The selective down-regulation of TIMP-2 and up-regulation of MMP-2 gene expression in atrium,especially the unbalanced change of the MMP-2 and TIMP-2 gene expression,could be one of the molecular mechanisms of atrial structural remodeling in patients with atrial fibrillation caused by rheumatic heart disease,which correlates with the initiation and maintenance of AF.

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Available abstract

Objective To investigate the matrix metalloproteinase-2 (MMP-2) and tissue inhibitor-2 of metalloproteinase (TIMP-2) mRNA expression in patients with atrial fibrillation caused by rheumatic heart disease and to evaluate the influence of MMP-2 and TIMP-2 expression on the progress of atrial structural remodeling.Methods 42 patients with chronic atrial fibrillation(AF) caused by mitral valve straitness of rheumatic heart disease were as experimental group, and l8 patients with sinus rhythm were as control group.Before operation,all of the above patients underwent transthoracic echocardiograph,and related clinical date were collected. Left atrial appendage (LAA) tissue samples were obtained from these patients during mitral valve replacement operation.Collagen fibers in left atrial appendage were observed by Masson trichrome stain. MMP-2 and TIMP-2 mRNA expressions were determined by reverse transcription polymerase chain reaction (RT-PCR).Results (1)Compare with control group,the content of collagen fibers in LAA increased significantly in patients with AF(P0.01). The content of collagen fibers in LAA was significantly correlated with left atrial dimension(r=0.788,P0.001)and the aera of mitral valve(r=-0.886, P0.05). (2)Compared with control group,the expressions of MMP-2 mRNA(P0.05) in the LAA tissue of the AF group was up-regulated significantly;the expressions of TIMP-2 mRNA(P0.05) in the LAA tissue of the AF group was down-regulated significantly. (3)The expressions of MMP-2 mRNA was significantly correlated with TIMP-2 mRNA(rp=-0.766,P0.001). The MMP-2 was significantly correlated with the content of collagen fibers in LAA(rp=0.869,P0.001).The TIMP-2 was significantly correlated with the content of collagen fibers in LAA(rp=-0.830,P0.001).Conclusion The selective down-regulation of TIMP-2 and up-regulation of MMP-2 gene expression in atrium,especially the unbalanced change of the MMP-2 and TIMP-2 gene expression,could be one of the molecular mechanisms of atrial structural remodeling in patients with atrial fibrillation caused by rheumatic heart disease,which correlates with the initiation and maintenance of AF.

Key concepts: Atrial fibrillation, Medicine, Internal medicine, Sinus rhythm, Matrix metalloproteinase, Cardiology, Heart disease, Tissue inhibitor of metalloproteinase

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