rpoB Gene Mutations in 55 Mycobacterium Tuberculosis Clinical Isolates

[Objective] To study characterizes of rpoB gene mutations of multi-drug resistant Mycobacterium tuberculosis(MTB) clinical isolates in Fujian,China.[Methods] Totally 55 MTB clinical isolates were recruited in this study.rpoB gene fragments including 81 bp rifampin resistance determining region(RRDR) of isolates were amplified and sequenced.[Results] No mutation was found in rpoB genes of the 10 sensitive isolates.Totally 21 mutations of various kinds were observed in 40 of 45 multi-drug resistant isolates,including 17 point mutations,2 insertions and 2 deletions.[Conclusions] rpoB gene mutations were detected in 88.9% of resistant isolates.The most frequent mutations are in codon 531,526 and 516,and the mutation rates are 47.1%,19.6% and 7.8% respectively.