Effects of Nitric Oxide in ABA-and Darkness-Induced Stomatal Closure of V. faba

[Objective] The role of NO in ABA-and darkness-induced stomatal closure in V.faba was studied.[Method] The abaxial surface of V.faba leaf was selected as the material,the research was carried out by epidermal strip bioassay and laser-scanning confocal microscopy.[Result] The results showed that both phytohormones ABA and darkness induced stomatal closure in V.faba.Furthermore,ABA and darkness could also improve the NO level of stomatal guard cells.ABA-and darkness-induced stomatal closure was largely prevented by 2,4-carboxyphenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide(cPTIO),a specific NO scavenger and NG-nitro-L-arg-methgl eater(L-NAME),an inhibitor of NO synthase(NOS) in mammalian cells that also inhibit plant NOS.The data indicate that NO is the common signaling molecule in ABA-and darkness-induced stomatal movement in V.faba.[Conclusion] The study can accumulate information for seeking network of the guard cell signal conduction pathway,and provide theoretical support for improving plant resistance and promoting agricultural production.