IGF-1 inhibited the MPP~+ induced apoptosis of PC12 cells via PI3K /Akt signaling pathway

Objective To investigate the protective mechanism of IGF-1 on MPP+induced neurotoxicity in PC12 cells. Methods PC12 cells impaired by MPP+( 250 μmol/L) were used as the cell model of Parkinson's disease. The cultured cells were divided into such groups:( 1) control group;( 2) MPP+group;( 3) IGF-1 group;( 4) IGF-1 + MPP+group;( 5) IGF-1 + MPP++ LY294002 group. After incubation for 24 h,methyl thiazolyltetrazolium( MTT) was used to assay the viability of the PC12 cells. After incubation for 4 h,Western blot was used to detect the expression level of Akt、phospho-Akt. Results( 1) 100 nmol /L IGF-1 protect PC12 cells from MPP+.( 2) The level of total-Akt had not changed after all the treatment,however,the level of phospho-Akt was higher in IGF-1 treated group than that treated with MPP+.Conclusion These findings indicate that IGF-1 protects against apoptosis in PC12 cells exposed to MPP+,the protective effect is associated with an up-regulation of phospho-Akt.