2013•Zhongguo yufang shouyi xuebaoRequires access

Development of loop-mediated isothermal amplification method for detection of Akabane disease virus

Zhang Ji-hon

Abstract

In the present study,a loop-mediated isothermal amplification(LAMP) method for detection of Akabane virus was developed using a set of primers designed according to the S gene of the virus.Under the optimized reaction conditions,the LAMP was specific to amplified the DNA from Akabane virus with a detection limit of 11.5 copies of the virus,and had no cross reaction with bluetongue virus,bovine viral diarrhea virus and infectious bovine rhinotracheitis virus.The established RT-LAMP technique in this study was rapid,sensitive and specific detection of Akabane virus,which is suitable for the application in field surveillance.

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Available abstract

Key concepts: Loop-mediated isothermal amplification, Virology, Virus, Biology, Porcine epidemic diarrhea virus, DNA, Genetics