Experimental study on curative effect of tea camellia seed oil on postmenopausal osteoporosis

Objective:To observe the effect of tea camellia seed oil on bone tissue and bone metabolism of ovariectomized rats,provide an experimental basis for preventing and treating postmenopausal osteoporosis with tea camellia seed oil.Methods:A total of 80 six-month healthy female SD rats were divided into four groups,20 rats in each group: control group,ovariectomized model group,ovariectomized model plus diethylstilbestrol group,and ovariectomized model plus tea camellia seed oil group.The rats in ovariectomized model plus diethylstilbestrol group were treated with subcutaneous injection of diethylstilbestrol for successive 12 weeks,the therapeutic dose was 300 μg/kg,twice a week;the rats in ovariectomized model plus tea camellia seed oil group were treated with pouring tea camellia seed oil into stomach for successive 12 weeks,the therapeutic dose was 5 ml/kg,once a day.The rats in the four groups were anesthetized at 12 weeks,then the blood samples of left heart were obtained to detect the levels of serum estradiol,progesterone,follicle-stimulating hormone(FSH),luteinizing hormone(LH),calcium,phosphorus,and alkaline phosphatase;then the rats were killed,proximal femoral sections were prepared to observe the structure of bone tissue;all the data were analyzed statistically.Results:Compared with control group,the levels of serum calcium,phosphorus,and estradiol in ovariectomized model group decreased significantly(P0.01),while the levels of alkaline phosphatase and FSH increased significantly(P0.01),bone sections showed obvious pathological changes of osteoporosis;compared with ovariectomized model group,the levels of serum calcium,phosphorus,and estradiol in ovariectomized model plus diethylstilbestrol group and ovariectomized model plus tea camellia seed oil group increased significantly(P0.01),while the levels of alkaline phosphatase and FSH decreased significantly(P0.01),bone sections showed that the pathological changes of osteoporosis were improved significantly.Conclusion:Tea camellia seed oil had therapeutic effect for osteoporosis in ovariectomized rats,and the effect was similar to that of diethylstilbestrol.