Overexpression of LMO3 Increases Proliferation of Glia Cells

C8 glia cells were cultured in DMEM with 10% FBS. Recombinant plasmid pcDNA4 / HisALMO3 was constructed and transfected into the C8 cells with the lipofectamine 2000. A stable C8 cell line overexpressing LMO3 was established to observe the direct effect of LMO3 on C8 cells. Parental C8 cells and parental C8 cells stably transfected with blank vector pcDNA4 / HisA were used as control groups. The cell growth was determined by MTT method and flow cytometry (FCM)was used to analyze the cell cycle in each group. The stably transfected cell line C8-LMO3 was confirmed by RT-PCR and Western blotting. After transfection with LMO3,C8 cells showed significant increase in cell proliferation when compared with the control groups. The percentage of S phase cells increased,whereas G0 / G1 phase cells decreased. Overexpression of LMO3 can stimulate proliferation by promoting glia cell into cell cycle.