Effects of ulinastatin on intestinal oxidative stress of rats with obstructive jaundice

Aim:To investigate the effects of ulinastatin(UIT) on intestinal oxidative stress of rats with obstructive jaundice.Methods:Seventy-two male SD rats were randomly and equally divided into sham operation group(Group A),obstructive jaundice group(Group B) and UIT treatment group(Group C).Rats in Group B and C were given choledochus ligation to establish the obstructive jaundice model.Group C was intraperitoneally injected UIT 40 000 IU/kg every day since the first day postoperation,while Group A and Group B were injected into equal amount normal saline.At Day 3,5,7 and 10 after operation,malondialdehyde(MDA) and superoxide dismutase(SOD) in the intestine tissue of six rats from every group were determined,plasm endotoxin were detected,the terminal ileum mucosa was observed under the light microscope,and the height of villus and mucosal thickness were examined with image analysis system.Results: Compared with those of Group A,MDA in the intestine tissue and the level of plasm endotoxin were higher,and SOD level was lower in Group B(P0.01).Compared with those of Group B,MDA in the intestine tissue and the level of plasm endotoxin were lower,and SOD level was higher in Group C(P0.05).The injury of intestinal mucosa was obriouse in Group B since Day 3 after operation,which aggravated with time changing,and it was lighter in Group C than that in Group B.The height of villus and mucosal thickness were shorter in Group B than that in Group A(P0.01),higher in Group C than that in Group B(P0.05);but there were no significant differences in indexes mentioned above between Group C and Group A at Day 3 after operation(P0.05).Conclusion: UIT may protect intestinal mucosal barrier and reduce endotoxemia in obstructive jaundice by resisting intestinal oxidative stress,and it is especially beneficial for the early change.