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2014•Zhongguo mianyixue zazhiRequires access

S100-B induce expression of IFN-γ via p38MAPK in Jurkat cells

Peng We

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Abstract

Objective: To investigate the role of S100-B on the expression of IFN-γ and associated signaling pathway in Jurkat cells. Methods: The expression of NF-κB was assessed by Western blot and the secretion of IFN-γ was tested by ELISA. In addition,total and phosphorylated p38MAPK was measured by Western blot,too. Results: S100-B enhanced the expression of NF-κB in Jurkat cells,S100-B induced Jurkat cells expressing IFN-γ,promoted p38MAPK phosphorylate. And,p38MAPK inhibitor inhibited S100-B-induced IFN-γ expression. Conclusion: S100-B promotes IFN-γ expression via p38MAPK in Jurkat cells which pre-stimulated by phytohemagglutinin( PHA).

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What this paper is about

Objective: To investigate the role of S100-B on the expression of IFN-γ and associated signaling pathway in Jurkat cells. Methods: The expression of NF-κB was assessed by Western blot and the secretion of IFN-γ was tested by ELISA. In addition,total and phosphorylated p38MAPK was measured by Western blot,too. Results: S100-B enhanced the expression of NF-κB in Jurkat cells,S100-B induced Jurkat cells expressing IFN-γ,promoted p38MAPK phosphorylate. And,p38MAPK inhibitor inhibited S100-B-induced IFN-γ expression. Conclusion: S100-B promotes IFN-γ expression via p38MAPK in Jurkat cells which pre-stimulated by phytohemagglutinin( PHA).

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Available abstract

Objective: To investigate the role of S100-B on the expression of IFN-γ and associated signaling pathway in Jurkat cells. Methods: The expression of NF-κB was assessed by Western blot and the secretion of IFN-γ was tested by ELISA. In addition,total and phosphorylated p38MAPK was measured by Western blot,too. Results: S100-B enhanced the expression of NF-κB in Jurkat cells,S100-B induced Jurkat cells expressing IFN-γ,promoted p38MAPK phosphorylate. And,p38MAPK inhibitor inhibited S100-B-induced IFN-γ expression. Conclusion: S100-B promotes IFN-γ expression via p38MAPK in Jurkat cells which pre-stimulated by phytohemagglutinin( PHA).

Key concepts: Jurkat cells, Western blot, Molecular biology, Phosphorylation, Secretion, Blot, Cell biology, Biology

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