Effects of VEGF Antisense Oligonucleotide on Expression of Survivin in Gastric Cancer Cells

Objective To observe the effects of VEGF antisense oligonucleotide on expression of survivin and growth in gastric cancer cells.Methods The VEGF-ASODN was synthesised artificially.After transfected with VEGF-ASODN,survivin protein was measured by Western-blot,apoptosis was detected by FCM,survival of cells was detected by MTT.Growth of cells was evaluated by growth curve.Results Transfected with VEGF-ASODN,it reduced the expression of survivin remarkably,increased apoptosis and decreased the survival and the growth of the cells.Conclusion VEGF-ASODN can reduce the expression of survivin,suppress the growth and increase the apoptosis of gastric cancer cell.