The effect of human fetal bone marrow stromal cells on the expansion of hematopoietic stem cells/progenitor cells in umbilical cord blood

Objective To study the expansion effect of human fetal bone marrow cells system on the hematopoietic stem cells in umbilical cord blood Methods Fetal bone marrow stromal cell layers (FBMSCL) and adult bone marrow stromal cell layers (ABMSCL) were established and their single and combined effects with hematopoietic growth factors (HGFs) on expansion of colony forming cells (CFCs), CD+34 cells and CD+34CD-38 cells in umbilical cord blood were observed at different time (day 10,21,30,38) Results (1) The expansion effect of FBMSCL on the CD+34 Cells and CFCs was better than ABMSCLs and was also better than ABMSCs when they were combined with HGFs (including umbilical cord blood plasma, SCF, IL3, IL6, IL1 , EPO, GCSF), respectively (2) During the CFCs peak on day 30, the percentage of early and midterm progenitors expanded by FBMSCL+HGFs was higher than that expanded by ABMSCL+HGFs (3) The CD+34CD-38 cells were three folds expanded by FBMSCL+HGFs and were not changed significantly by ABMSCL+HGFs When they were expanded on the fifth to sixth week, CD+34CD-38 cells were not wasted up There were 75% and 25% CD+34CD-38 cells supported by FBMSCL+HGFs and ABMSCL+HGFs, respectively Conclusion Human fetal bone marrow stromal cells system is one of the ideal expansion systems of hematopoietic stem/progenitor cells in umbilical cord blood