Effects of NF-κB inhibitor on PMN apoptosis in lung injury associated with paraquat poisoning of rats

Objective:To investigate the effects of NF-κB inhibitor(PDTC) on PMN apoptosis in lung injury associated with paraquat poisoning of rats. Methods:120 Rats were randomly divided into four groups:C group (NS 3ml/kg,ip),PC group (PDTC120mg/kg,ip and after half an hour NS 3ml/kg,ip),L group ( 2% PQ 25mg/kg,ip),P+L group (PDTC120mg/kg,ip and after half an hour 2% PQ 25mg/kg,ip). L group and P+L group were divided into 1d、3d、5d sub group further,each group had 16 rats. Used optical microscope to observe the pathologic change of lungs.The level of TNF-α in serum was detected. The expression of NF-κB P65 in PMN of BALF was determined. The content of IκB α of cytoplasm protein was determined.The ratio of PMN apoptosis was assaied.Results:The degree lung injury in L group was higher than that in C group and PC group. But in P+L group the degree of lung injury was lighter.The content of TNF-α in L group was higher than that in P+L group. The ratio of PMN apoptosis in L group was lowest(3.52±0.34%).The ratio of PMN apoptosis in P+L group raised. Conclusion:Paraquat poisoning leads to acute lung injury in rats.After using of NF-κB inhibitor(PDTC),the process of PMN apoptosis regained normal and the lung injury associated with paraquat poisoning getslighter.