Protective effect of Shen-fu injection on liver injury induced by intestinal ischemia reperfusion in rats

OBJECTIVE To investigate protective effect of Shen-fu injection(SFI) on liver injury induced by intestinal ischemia reperfusion in rats.METHODS40 male Wistar rats were randomly divided into four groups: Sham group,intestinal I/R(II/R) group,low dosage of SFI group(SF-5 group,5 mL·kg-1) and high dosage of SFI(SF-10,10 mL·kg-1) group.Intestinal I/R model were established by clamping superior mesenteric artery for 60 min and reperfusion for 120 min.SFI was administered(ip) before reperfusion.In Sham group,superior mesenteric artery was only isolated without clamping.Blood samples,intestinal and liver tissue samples were collected after reperfusion lasting 120 min.MDA level in intestinal,AST,ALT level in serum and MDA,TNF-α level in liver tissue were determined.Histomorphology changes of intestinal and liver tissue were observed with light microscope.The NF-κB activity in liver cells was determined by Western Blot analysis.RESULTSCompared with Sham group,MDA level in intestinal,AST and ALT level in serum,MDA,TNF-α and NF-κB levels in liver tissue were increased in II/R group(P0.05).Histomorphology of intestinal tissue and liver tissue in II/R group was destroyed obviously,as seen by light microscope.Compared with II/R group,SFI decreased intestinal MDA level,AST,ALT in serum and MDA,TNF-α,NF-κB levels in liver tissue(P0.05),attenuated the histomorphological injury of liver tissue.CONCLUSIONSFI has a protective effect on liver injury induced by intestinal ischemia reperfusion by attenuating NF-κB activation in liver tissue.