Effect of ω-3PUFAs on the TNF-α,IL-1β and IL-6 expression on acute lung injury in young rats induced by endotoxin

Objective To investigate the effects ofω-3polyunsaturated fatty acids(ω-3PUFAs)to LPS-induced rat lung(ALI)of inflammatory:TNF-α,IL-1βand IL-6. Methods Sixty young SD rats were divided into control group,LPS group and omegagroup and then used normal saline or different fat emulsions for seven days.The rat model of ALI was established by instill LPS.Those groups were sacrificed at 8hours after intratracheal introduction of LPS.Each lung tissue section's pathologic changes was observed,meanwhile the concentrations of TNF-α,IL-1βand IL-6in BALF were measured by enzyme-linked immunospecific assay(ELISA). Results 1)Pulmonary histopathology:ALI modle groups showed inflammatory cells infiltrating around the bronchus and the vascular,even bleeding and more apoptosis cells.Omegagroup was significantly lighter than LPS group(P0.01).2)Compared with control group,the lung indexes and pathological scores of ALI model groups were significantly higher(P0.05).3)The levels of TNF-α,IL-1βand IL-6in BALF of LPS group were significantely elevated than omegagroup(both P0.05). Conclusion 10%Omegaven can relieve inflammation by downregulating the concentrations of TNF-α,IL-1βand IL-6and reduce lung injury.