Effect of astragalus polysaccharides on expression of NF-κB and IκB in renal tissue of diabetic rats with deficient kidney yang

[Objective]To investigate the effect of astragalus polysaccharides(APS)on the expression of nuclear factor kappa B(NF-κB) and inhibitor protein of nuclear factor κB(IκB) in the renal tissue of diabetic rats with deficient kidney yang.[Methods] Male SD rats were divided into 4 groups: the normal control group,the diabetic nephropathy group,benazepril treated group and APS treated group.At the end of 8th week,the kidneys were taken out from all the rats to measure the mRNA expression of NF-κB and IκB by the method of RT-PCR.Besides,the ratio of renal weight to body,blood sugar(BS),blood fat and renal function was measured respectively. [Results]As compared with benazepril,APS inhibited the expression of NF-κB mRNA and increased that of IκB mRNA,decreased the ratio of renal weight to body,BS and blood fat,improved renal function more significantly.[Conclusion] APS can up-regulate the expression of IκB mRNA,inhibit the over-expression of NF-κB mRNA and delay the development of diabetic nephropathies with deficient kidney yang.