INFLUENCE OF OXYGEN CONCENTRATION ON THE PRODUCTION OF PEROXIDASES BY PHANEROCHAETE CHRYSOSPORIUM
Ke Shi
Abstract
Ke Shi
Abstract
Oxygen concentration had obvious influences on the production of peroxidases by Phanerochaete chrysosporium . Under limited carbon source culture condition,oxygen concentration in the growth period had little influence on the production of lignin peroxidase,but it had obvious influences in the second metabolism period in which peroxidases were produced.It was found that 21?% O 2(in air)was the optimal oxygen condition on production of peroxidases (including both lignin peroxidase and Mn dependent peroxidase ).Further increase of oxygen concentration depressed the production of peroxidases,because more extracellular protease which was harmful to peroxidases would be produced.At the optimal oxygen concentration (in air 21?% O 2),the maximal lignin peroxidase activity was 360?U/L under limited carbon source culture condition.
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Oxygen concentration had obvious influences on the production of peroxidases by Phanerochaete chrysosporium . Under limited carbon source culture condition,oxygen concentration in the growth period had little influence on the production of lignin peroxidase,but it had obvious influences in the second metabolism period in which peroxidases were produced.It was found that 21?% O 2(in air)was the optimal oxygen condition on production of peroxidases (including both lignin peroxidase and Mn dependent peroxidase ).Further increase of oxygen concentration depressed the production of peroxidases,because more extracellular protease which was harmful to peroxidases would be produced.At the optimal oxygen concentration (in air 21?% O 2),the maximal lignin peroxidase activity was 360?U/L under limited carbon source culture condition.
Key concepts: Phanerochaete, Peroxidase, Chemistry, Lignin peroxidase, Chrysosporium, Lignin, Oxygen, Biochemistry