ERβ regulates osteogenic potential of osteoblast through regulation of OPG/RANK/RANKL signaling in mice

【Objective】 At the cellular level,to study whether the ERβ regulates the secretion and expression of the cytokine OPG and RANKL and the osteogenic capability of mice osteoblasts.【Methods】 The mice osteoblastic cell lines MC3T3-E1 is divided into 3 groups,tow of which groups were transfected with recombinant plasmid ERβ RNAi and negative control plasmid ERLRNAi,group 3 was the control group,the same amount of PBS instead of plasmids.3 groups of cells cultured under the same conditions.In each group,OPG and RANKL expression,alkaline phosphatase(ALP) activity and the calcium nodules were detected.SPSS 16.0 software was used.【Results】 The results indicate that the OPG Ct value was relatively lower,gene expression was more enhanced.Meanwhile,the Ct value of the RANKL is higher in RNAi group than in other two groups,gene expression was more reduced.ALP activity in the RNAi group was higher than those in other groups.The calcium nodules formed in RNAi group were a larger number and larger volume compared with the control group or negative control group.【Conclusion】 According to the results of silencing,we can reversely infer that ERβ expression may inhibit the proliferation,differentiation and maturation of osteoblasts,with reducing the secretion of ALP,the expression of OPG and increasing the expression of RANKL,thereby inhibiting the formation of new bone.