2007•Journal of Nuclear Agricultural SciencesRequires access
CYTOLOGICAL EFFECTS OF ~(60)Co γ-RAYS IRRADIATION ON SEEDS OF Medicage sative L.
Rubing Yang, Xiangling Xu, LI Ji-lin, Wang JiHua, Zhang Yuexue, Zhang Hong-Qiang, Weibo Han, Tang Feng-lan, Liu JieLin, Daoming Li, Song Ruochao, Shen Zhongbao
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Abstract
Seeds of three genotypes Medicago sative L.were irradiated by 0,1000,1500 and 2000Gy()~(60)Co γ-rays,comparison was made among different genotypes at same dosage,and the other is the same genotype at different dosage.The effects of irradiation on root tip cell in Medicago sative L.were studied.The results showed that the irradiation of γ-rays inhibited mitoses and produced various aberrations,such as single micronucleus,double micronucleus,multi-micronucleus,small micronucleus,chromosome fragment,chromosome conglutination,single bridge,double bridge,multi-bridge,dissociative chromosome,lagging bridge in root tip cells of Medicago sative L.Under the same irradiation dosage,Longmu 803 genotype is the most sensitive one.The higher the γ-rays irradiation dosages,the higher the rate of aberration in 0 to 2000Gy,at 2000Gy irradiation dosage,all the aberration rates reach the top,resulting in the relative higher biological effects of γ-rays irradiation of high dosage.