Apoptosis and Fas/FasL expression of hepatic cells in rat precancerous lesion of liver

Objective To investigate the apoptosis and Fas/FasL expression of liver cells during liver precancerous lesion,and to provide experimental basis for treatment and prevention of liver disease.Methods Rats were randomly divided into control group and experiment group.The rat liver precancerous lesion models were established by injecting diethylnitrosamine (DEN) in intraperitoneal.Alanine amiotransferase(ALT),aspartate aminotransferase(AST),γ-glutamyl transpeptadase(γ-GT),α-L-fucosidase(AFU),alpha-fetoprotein(AFP) and carcinoembryonic antigen(CEA) content in serum,were measured by biochemical technology.Liver pathological characteristics were examined by microscope.The apoptosis of liver cells was detected by DNA agarose electrophoresis.The localization and expression of Fas and FasL were identified by immunohistoehemistry. Results The liver tissue was normal in the control group.The liver cell appeared engorge;Focal necrosis and inflammatory cell infiltration were discovered in hepatic lobules;Fibroplasia and regeneration gradually occurrenced in model group.Compared with control group,the ALT,AST,γ-GT and AFU content in serum in model group 1-8 weeks and 9-12 weeks were increased(P0.01);The ALT,AST,γ-GT and AFU content in serum of model group during 9-12 weeks were significantly higher than 1-8 weeks(P0.05),and AFP and CEA content in serum remained unchanged in model group.The hepatocyte apoptosis was not found in the control group.The obvious DNA ladder was observed in liver cell in the model group.The Fas and FasL expression were not found in the control group.Fas and FasL expressed mainly in the liver cell cytoplasm in the model group.Conclusion The increase of liver cell apoptosis may be closely related with the increase of Fas/FasL expression in the rat liver precancerous lesion.The increase of the hepatocyte apoptosis may be reasons of liver damage aggravation in rat liver precancerous lesion.