Effect of hematoporphyrin derivatives on the permeability of erythrocyte membrane

After adding NaNO2(the methe moglobin former) to mice erythrocyte suspension, the permeability of the red cell membrane was determined by measuring the amount of methemoglobin formation, and the effect of hematoporphyrin derivative (HPD) on the red cell membrane was investigated. HPD may injury the erythrocyte membrane and increase the transference of NaNO2 into red cells. With the increase of the concentration of HPD, the methemoglobin formation was increased as shown inthe equation: where C = concentration of HPD in mg / ml.The HPD-treated red cell membrane becomes more intensively injured by light irradiation and the re-lationship between MetHb formation and the time of irradiation is as follows: Where t = irradiation time (h).The HPD-treated red cell is also sensitive to NaNO2. The MetHb formation was varied with the concentration of NaNO2 in the equation ofMetHb % = 1.823 e71.08cwhere c = concentration of NaNO2. The permeability of HPD-treated red cells to NaNO2 is also varied with temperature.