Effect of Dihydroartemisinin on Apoptosis and Cell Growth Inhibitory in Human Colon Cancer HCT-116 Cells

Objective:To investigate the effect of dihydroartmisinin on apoptosis and cell growth inhibitory in Human Colon cancer Hct116 cells cultured in vitro.Methods:HCT-116 cells were treated with DHA at various concentrations and determined for proliferation inhibiting rate by MTT method.The apoptosis induction was examined by flow cytometry.The expression of the Bcl-2,bax,cleavad-PARP which associated with cell apoptosis were measured by Western-Blotting.Results:DHA showed significantly time-and dosage-dependent inhibitouy effect on the proliferation of HCT-116 cells,the IC50 values were 14.18 μmol/L.Flow cytometry demonstrated that the percentage of the cells in Sub-G0 phase increase remarkably after treated with DHA.Western-Blotting also revealed that DHA down-regulated Bcl-2,up-regulated Bax and cleavad-PARP.Conclusions:DHA can induce apoptosis and inhibit the proliferation of HCT-116 cells significantly.