2006•Basic & Clinical MedicineRequires access
Increased P53 expression in the lung tissue of rats with chronic hypoxic pulmonary hypertension
Peng Li-jing, LI Zhi-chao, Wěi Li, Bo Zhang, Yi Liu, Qi Zhang, Ying Luo
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Abstract
Objective To delinate the expression of P53 in the lung tissue of rats with chronic hypoxic pulmonary hypertension(HPH).Mehtods The chronic HPH rat models were duplicated by low-pressure and hypoxic approaches.The expression of P53 in the lung tissue of HPH rats was explored by ABC immunohistochemistry(IHC).The number of apoptosis cells were measured by Hoechst staining.Results IHC staining showed that the positive cell of P53 in lung tissue was increased in hypoxic group,especially cells in the wall of pulmonary arteriolar,and the expression intensity of P53 in hypoxic hypoxic 2 weeks groupcontrol group.Hoechst staining showed that the number of apoptosis cell in the lung tissue was also increased.The number of apoptosis cells in hypoxic was significantly more than those in 2 weeks hypoxic group and control group.Conclusions In the process of chronic HPH rats,both the proliferation and aopotosis in the wall of pulmonary arteriolar are increased,and the expression of P53 in the lung tissue has an increasing tendency.The activity of P53 may play a regulation role in pulmonary arteriolar reconstruction in chronic HPH rats.