Effect of intra-uterine actute ischemia/reperfusion on superoxide dismutase and malondialdehyde in fetus rat kidney

Objective To study the changes of superoxide dismutase and malondialdehyde in fetus rat kidney during actute ischemia/reperfusion injury and to explore pathophysiologic mechanism of renal ischemia/reperfusion injury in fetal rats.Methods The pregnant 21 days Wistar rats were anesthetized intraabdominaly.After two-horn uterus and vessels supplying uterus and ovary were exposed.arterial clamp occluded one side of vessels. Another side was regarded as sham operation group. The measured time after ischemia was 10min and 30min. The measured time afte reperfusion time was 30min. 2hr.6hr and 24hr. The contents of superoxide dismutase (SOD) and malondialdehyde (MDA) in both ischemia and reperfusion period were detected.Results (1)The activity of SOD weakened gradually with the extension of ischemia period. especially in 30minutes' ischemia group. It decreased progressively during reperfusion and reach minimum after 6hours' reperfusion.Then it began to increased.There was no difference between sham operation group and observed group in reperfused 24 hours.These changes showed significance in reperfusion group after 30 minutes ischemia.(2)The contents of MDA increased little by little in ischemia and reperfusion period especially at 30 minutes' ischemia group.Conclusions When there is simply ischemia.the activity of decreased SOD and increased MDA indicated that free radicals could be produced in this stage. After reperfusion.changes of SOD and MDA prove that free radicals injury may be one of mechanisms of renal injury.