Protective effects of ATP regulated potassium channel opener on isolated lung

Objective To investigate the protective effects of pinacidil,an ATP regulated potassium channel opener,on isolated lung.Methods Fifteen hybrid gray rabbits were devided into 3 groups randomly.Isolated,reperfused ventilateing rabbit lung models were used.Control group(n=6) underwent immediate reperfusion for 2 h.Ischemic group(n=6) were flushed with LPD solution + DMSO,stored at 4℃ for 8 h and then reperfused for 2 h.Pinacidil protected group(n=6) were flushed with LPD+ pinacidil 100 μmol·L-1(soluted in DMSO),stored at 4℃ for 8 h and then reperfused for 2 h.Every 30 min,blood was taken from the pulmonary artery and left atrium for blood gas analysis to evaluate the function of lungs.The histologic injury of lung samples was observed.The wet-to-dry ratios and the content of SOD,TNF-α,IL-8 in the homogenate of lung were measured.Results The ΔPO2 and SOD in pinacidil group were significantly higher than those in ischemic group(all P0.05).The histologic injury in pinacidil group was less than that in ischemic group.The concentration of TNF-α and IL-8 in pinacidil group were significantly lower than those in ischemic group(P0.05).Conclusions The protective effects of pinacidil in conjunction with LPD solution on isolated rabbit lung are better for a period of 8 h preservation.