Comparison among several methods for apoptosis examination by Flow Cytometry

Objective:To explore the methods of using Flow Cytometry to examine the apoptosis.Methods:Flow Cytometry was used to examine the DNA content,the phosphatidyl serine,the mitcchondrial membrane potential and the calcium ion,the apoptosis was analyzed.Results:The DNA content had some deflexion.The phosphatidyl serine examination can reflect the early and advanced stage apoptosis punctually.The mitcchondrial membrane potential and the calcium ion examination can reflect the physiological index of apoptosis cell.But if combined with morphology the analysis will be more objective.Conclusion:Through comparing empirical method,we find more precise methods of using the Flow Cytometry to examine the apoptosis in the future.