Breast fibroadenoma and breast invasive ductal carcinoma: a comparative study using ~1HNMR-based serum metabonomics

Objective To explore the serum metabonomic difference between breast fibroadenoma patients and breast invasive ductal carcinoma patients using nuclear magnetic resonance1H(1HNMR) spectroscopy,and to discuss the possibility of using it for differentiation of benign and malignant breast tumor before operation. Methods Eight clinically diagnosed breast fibroadenoma patients and 8 breast invasive ductal carcinoma patients were recruited and their sera were collected;1HNMR was used to obtain the metabonomic characteristics. The data were processed using Simca-P software and analyzed with principle component analysis( PCA)and orthogonal signal correction partial least squares discrimination analysis( OPLS-DA). Results PCA and OPLS-DA analyses showed great differences in serum metabonomics between breast fibroadenoma and breast invasive ductal carcinoma. Compared with breast fibroadenoma patients, patients with invasive ductal carcinoma had higher levels of lipid, lactate and phosphocholine lglycerolphospholine,and lower levels of glutamine,taurine,creatine,and glucose. Conclusion Distinct difference exists in serum metabonomic profiles between breast adenoma patients and breast invasive ductal carcinoma patients,which may serve as the potential biomarkers to differentiate malignant tumors from benign ones in clinic.