2002•Zhongguo renshougonghuanbing zazhiRequires access
Fused expression of hantaan virus partial NP and glycoprotein G1 in insect cells
Yan Yan
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Abstract
Aim To obtain the fused expression of hantaan virus glycoprotein G1 and partial NP by using the Bac to Bac baculovirus expression system Methods A recombinant baculovirus expression vector pFBD G1S0 7 carrying chimeric gene G1S0 7 of hantaan virus was constructed The gene was inserted into shuttle plasmid(Bacmid) in E coli DH10Bac with the help of Tn7 transposition system The recombinant baculovirus was selected and the fusion protein was expressed in insect cells The expression was identified by ELISA,immunofluorescence and Western blot Results The recombinant baculovirus containing the chimeric gene G1S0 7 was constructed successfully and could express the fusion protein in insect cells This protein could be recognized by the hantaan virus nucleoprotein specific mAb and glycoprotein G1 specific mAb Most production was within cells Conclusion The biologically active fusion protein G1S0 7 has been expressed in insect cells It could be used for further research on its immunological characteristic